Purification and partial characterization of nonspecific lipase from rat pancreas
作者： Phillip W. Albro , Barbara J. Corbett , Ann D. Latimer
DOI: 10.1016/0005-2760(76)90025-4
关键词: Enzyme 、 Phosphate 、 Size-exclusion chromatography 、 Titration 、 Lipase 、 Micelle 、 Chromatography 、 Chemistry 、 Imidazole 、 Acetone 、 Biochemistry 、 Biophysics 、 Endocrinology
摘要: Abstract Nonspecific lipase (also referred to as micelle and secondary ester hydrolase) has been purified electrophoretic homogeneity starting from acetone powder of rat pancreas. The enzyme is found have a molecular weight (gel filtration) 64 000 ± 2000, an equivalent (titration with E-600) 65 000. seen be very sensitive inhibition by organophosphates but resistant quinine. Evidence for the presence sulfhydryl imidazole groups essential activity presented, some observations on substrate specificity are made. appears lack phosphate lipids, unstable under conditions low ionic strength and/or exposure 2-mercaptoethanol.
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sci-hub.se 参考文章(18)
George Kalnitsky, John P. Hummel, Christa Dierks, Some factors which affect the enzymatic digestion of ribonucleic acid. Journal of Biological Chemistry. ,vol. 234, pp. 1512- 1516 ,(1959) , 10.1016/S0021-9258(18)70040-9
J. Hyun, C.R. Treadwell, G.V. Vahouny, Pancreatic juice cholesterol esterase: Studies on molecular weight and bile salt-induced polymerization☆ Archives of Biochemistry and Biophysics. ,vol. 152, pp. 233- 242 ,(1972) , 10.1016/0003-9861(72)90211-1
J Hyun, H Kothari, E Herm, J Mortenson, C R Treadwell, G V Vahouny, Purification and properties of pancreatic juice cholesterol esterase. Journal of Biological Chemistry. ,vol. 244, pp. 1937- 1945 ,(1969) , 10.1016/S0021-9258(18)91769-2
Lars A. Carlson, Chromatographic separation of serum lipoproteins on glass powder columns Clinica Chimica Acta. ,vol. 5, pp. 528- 538 ,(1960) , 10.1016/0009-8981(60)90064-4
K Weber, M Osborn, The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis Journal of Biological Chemistry. ,vol. 244, pp. 4406- 4412 ,(1969) , 10.1016/S0021-9258(18)94333-4
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
F.H. Mattson, R.A. Volpenhein, Hydrolysis of primary and secondary esters of glycerol by pancreatic juice Journal of Lipid Research. ,vol. 9, pp. 79- 84 ,(1968) , 10.1016/S0022-2275(20)43146-3
A Vandermeers, J Christophe, α-Amylase et lipase du pancreas de rat. Purification chromatographique, recherche du poids moleculaire et composition en acides amines Biochimica et Biophysica Acta (BBA) - Protein Structure. ,vol. 154, pp. 110- 129 ,(1968) , 10.1016/0005-2795(68)90264-X
Phillip W. Albro, Ann D. Latimer, Pancreatic nonspecific lipase, an enzyme highly specific for micelles. Biochemistry. ,vol. 13, pp. 1431- 1439 ,(1974) , 10.1021/BI00704A018
A. Chalvardjian, E. Rudnicki, Determination of lipid phosphorus in the nanomolar range. Analytical Biochemistry. ,vol. 36, pp. 225- 226 ,(1970) , 10.1016/0003-2697(70)90352-0