Exploiting 2A Peptides to Elicit Potent Neutralizing Antibodies by a Multi-Subunit Herpesvirus Glycoprotein Complex
作者： Felix Wussow , Flavia Chiuppesi , Zhuo Meng , Joy Martinez , Jenny Nguyen
DOI: 10.1016/J.JVIROMET.2017.10.006
关键词:
摘要: Neutralizing antibodies (NAb) interfering with glycoprotein complex-mediated virus entry into host cells are thought to contribute the protection against herpesvirus infection. However, using complexes as vaccine antigens can be complicated by necessity of expressing multiple subunits simultaneously allow efficient complex assembly and formation conformational NAb epitopes. By a novel bacterial artificial chromosome (BAC) clone clinically deployable Modified Vaccinia Ankara (MVA) vector exploiting ribosomal skipping mediated 2A peptides, MVA vectors were generated that expressed self-processing human cytomegalovirus (HCMV) pentamer (PC) composed gH, gL, UL128, UL130, UL131A. These 2A-linked HCMV PC efficiently cleaved transported cell surface protein forming neutralizing In addition, vaccination mice only two immunizations these resulted in potent responses remained stable over period at least six months. This method eliciting 2A-linked, could develop candidate may serve template facilitate development subunit strategies other herpesviruses.
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