Assembly of vaccinia virus: the second wrapping cisterna is derived from the trans Golgi network.
作者： M Schmelz , B Sodeik , M Ericsson , E J Wolffe , H Shida
DOI: 10.1128/JVI.68.1.130-147.1994
关键词: Orthopoxvirus 、 Membrane protein 、 Immunoelectron microscopy 、 Cisterna 、 Virion membrane 、 Endocytic cycle 、 Biology 、 Endosome 、 Cell biology 、 Golgi apparatus
摘要: During the assembly of vaccinia virus, intracellular mature virus becomes enwrapped by a cellular cisterna to form enveloped (IEV), precursor extracellular (EEV). In this study, we have characterized origin wrapping electron microscopic immunocytochemistry using lectins, antibodies against endocytic organelles, and recombinant viruses expressing proteins which behave as Golgi resident proteins. No labelling for marker could be detected on membrane. However, membrane labelled significantly trans network (TGN) protein. The recycling pathway from endosomes TGN appears greatly increased following infection, since significant amounts fluid-phase tracers were found in lumen TGN, complex, cisternae. Using immunoelectron microscopy, localized VV-p37, VV-p42, VV-p21, VV-hemagglutinin (VV-HA) large cisternae, outer membranes IEV, outermost EEV. bulk VV-p42 whereas VV-HA was also plasma endosomes. Collectively, these data argue that enriched facilitate event responsible formation IEV.
elsevier.com
sci-hub.st 参考文章(55)
G Griffiths, S Pfeiffer, K Simons, K Matlin, Exit of newly synthesized membrane proteins from the trans cisterna of the Golgi complex to the plasma membrane. Journal of Cell Biology. ,vol. 101, pp. 949- 964 ,(1985) , 10.1083/JCB.101.3.949
L. Orci, M. Ravazzola, M. Amherdt, D. Louvard, A. Perrelet, Clathrin-immunoreactive sites in the Golgi apparatus are concentrated at the trans pole in polypeptide hormone-secreting cells. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 82, pp. 5385- 5389 ,(1985) , 10.1073/PNAS.82.16.5385
G Griffiths, R Back, M Marsh, A quantitative analysis of the endocytic pathway in baby hamster kidney cells. Journal of Cell Biology. ,vol. 109, pp. 2703- 2720 ,(1989) , 10.1083/JCB.109.6.2703
Hisatoshi Shida, Nucleotide sequence of the vaccinia virus hemagglutinin gene Virology. ,vol. 150, pp. 451- 462 ,(1986) , 10.1016/0042-6822(86)90309-0
Richard G.W. Anderson, Ravindra K. Pathak, Vesicles and cisternae in the trans golgi apparatus of human fibroblasts are acidic compartments Cell. ,vol. 40, pp. 635- 643 ,(1985) , 10.1016/0092-8674(85)90212-0
B Sodeik, R W Doms, M Ericsson, G Hiller, C E Machamer, W van 't Hof, G van Meer, B Moss, G Griffiths, Assembly of vaccinia virus: role of the intermediate compartment between the endoplasmic reticulum and the Golgi stacks. Journal of Cell Biology. ,vol. 121, pp. 521- 541 ,(1993) , 10.1083/JCB.121.3.521
Ian S. Trowbridge, Roland A. Newman, Derrick L. Domingo, Carol Sauvage, Transferrin receptors: structure and function. Biochemical Pharmacology. ,vol. 33, pp. 925- 932 ,(1984) , 10.1016/0006-2952(84)90447-7
Cecilia Bucci, Robert G. Parton, Ian H. Mather, Henk Stunnenberg, Kai Simons, Bernard Hoflack, Marino Zerial, The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway. Cell. ,vol. 70, pp. 715- 728 ,(1992) , 10.1016/0092-8674(92)90306-W
Gareth Griffiths, Bernard Hoflack, Kai Simons, Ira Mellman, Stuart Kornfeld, The mannose 6-phosphate receptor and the biogenesis of lysosomes Cell. ,vol. 52, pp. 329- 341 ,(1988) , 10.1016/S0092-8674(88)80026-6
Yukiko Goda, Suzanne R. Pfeffer, Selective recycling of the mannose 6-phosphate/IGF-II receptor to the trans Golgi network in vitro Cell. ,vol. 55, pp. 309- 320 ,(1988) , 10.1016/0092-8674(88)90054-2