摘要: The large size of poxvirus virions (approx 250 x 350 nm) makes them ideal candidates for microscopic studies. Recombinant vaccinia viruses that express a viral envelope-specific, green fluorescent protein (GFP) chimera produce enveloped fluoresce green. This labeling allows the live, real-time study egress using variety techniques. methods presented here describe how to image movement intracellular are labeled with time-lapse laser scanning confocal microscopy. Details also provided analyzing images obtained and converting into QuickTime movies suitable presentation.
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