Alterations in phosphoproteome under salt stress in Thellungiella roots

作者: YiJun Zhou , Fei Gao , XiaoFeng Li , Jun Zhang , GenFa Zhang

DOI: 10.1007/S11434-010-4116-1

关键词:

摘要: High salinity stress is a major environmental factor that limits plant’s distribution and productivity. An Arabidopsis-related halophyte, Thellungiella halophila, an emerging model system used for plant abiotic tolerance research. Previous studies have suggested protein phosphorylation has crucial role in the high response plants. However, phosphoproteome differential expression under halophytes not been well studied. In this report, was analyzed roots. Twenty-six putative phosphoproteins were found to changed pattern at post-translational level. Twenty of these identified by mass spectrometric analysis, including 18 upregulated two downregulated phosphoproteins. These proteins involved variety cellular processes, such as signal transduction, ROS detoxification, energy pathway, synthesis folding. While most salt-responsive are known salt-stress-related proteins, some them previously reported. Our results provide only new insights into salt responses but also good foundation further investigation salinity-regulated

参考文章(40)
H.A. Kang, H.G. Schwelberger, J.W. Hershey, Translation initiation factor eIF-5A, the hypusine-containing protein, is phosphorylated on serine in Saccharomyces cerevisiae. Journal of Biological Chemistry. ,vol. 268, pp. 14750- 14756 ,(1993) , 10.1016/S0021-9258(18)82396-1
Barbara Marzocchi, Lucia Ciccoli, Chiara Tani, Silvia Leoncini, Viviana Rossi, Luca Bini, Serafina Perrone, Giuseppe Buonocore, Hypoxia-induced post-translational changes in red blood cell protein map of newborns. Pediatric Research. ,vol. 58, pp. 660- 665 ,(2005) , 10.1203/01.PDR.0000180545.24457.AC
Salvatore Scacco, Rosaria Vergari, Richard C. Scarpulla, Zuzana Technikova-Dobrova, Annamaria Sardanelli, Rossana Lambo, Vito Lorusso, Sergio Papa, cAMP-dependent Phosphorylation of the Nuclear Encoded 18-kDa (IP) Subunit of Respiratory Complex I and Activation of the Complex in Serum-starved Mouse Fibroblast Cultures Journal of Biological Chemistry. ,vol. 275, pp. 17578- 17582 ,(2000) , 10.1074/JBC.M001174200
Elizabeth A. Veal, Victoria J. Findlay, Alison M. Day, Stephanie M. Bozonet, Jennifer M. Evans, Janet Quinn, Brian A. Morgan, A 2-Cys Peroxiredoxin Regulates Peroxide-Induced Oxidation and Activation of a Stress-Activated MAP Kinase Molecular Cell. ,vol. 15, pp. 129- 139 ,(2004) , 10.1016/J.MOLCEL.2004.06.021
Fei Gao, YiJun Zhou, LingYun Huang, DaCheng He, GenFa Zhang, Proteomic analysis of long-term salinity stress-responsive proteins in Thellungiella halophila leaves Science Bulletin. ,vol. 53, pp. 3530- 3537 ,(2008) , 10.1007/S11434-008-0455-6
Tingfang Yi, Gaofeng Dong, Zenglan Wang, Jiaxu Li, Identification and Functional Analysis of Phosphoproteins Regulated by Auxin in Arabidopsis Roots Journal of Plant Biology. ,vol. 52, pp. 65- 72 ,(2009) , 10.1007/S12374-008-9005-7
W. G. Li, S. Komatsu, Cold stress-induced calcium-dependent protein kinase(s) in rice (Oryza sativa L.) seedling stem tissues Theoretical and Applied Genetics. ,vol. 101, pp. 355- 363 ,(2000) , 10.1007/S001220051491
Birte Schulenberg, Terrie N. Goodman, Robert Aggeler, Roderick A. Capaldi, Wayne F. Patton, Characterization of dynamic and steady-state protein phosphorylation using a fluorescent phosphoprotein gel stain and mass spectrometry. Electrophoresis. ,vol. 25, pp. 2526- 2532 ,(2004) , 10.1002/ELPS.200406007
Zhijun Li, Haruko Onodera, Masashi Ugaki, Hiroshi Tanaka, Setsuko Komatsu, Characterization of calreticulin as a phosphoprotein interacting with cold-induced protein kinase in rice. Biological & Pharmaceutical Bulletin. ,vol. 26, pp. 256- 261 ,(2003) , 10.1248/BPB.26.256