Expression analysis of the PMP22 gene in glioma and osteogenic sarcoma cell lines.

摘要: Previously we reported the amplification of peripheral myelin protein 22 (PMP22) gene in cell lines human osteogenic and glioma tumors. PMP22 normally is expressed at high levels Schwann cells nervous system suggested to function as a structural sheath. One most common inherited neuropathies, Charcot-Marie-Tooth Type 1A (CMT1A), associated with duplication 1.5-Mb DNA region on chromosome 17p11.2 – p12 containing PMP22. On other hand, identical gas3, whose expression induced growth-arresting NIH3T3-fibroblasts thought play role proliferation. The precise gas3/PMP22 remains be determined. Here show that tumor RH30 SF763 amplified including comprises whole CMT1A region. We could prove by reverse transcriptase-polymerase chain reaction (RT-PCR) discovered an unusual transcript these lines. Western blot analyses resulted detection 22-kDa PMP22-specific antibody 558/2 exclusion zero (MPZ) J. Neurosci. Res. 58:624–631, 1999. © 1999 Wiley-Liss, Inc.