Endotoxin filtration and immune stimulation improve survival from gram-negative sepsis.

摘要: Abstract Polymyxin B, when bound to a polystyrene fiber (PMX-F), has been used experimentally as an extracorporeal blood filter reduce serum lipopolysaccharide levels, which are believed be responsible for physiologic alterations in the septic state. To validate our theory that combination of PMX-F, systemic antibiotics, and immune stimulation would improve survival, 78 rats were given intravenous doses Escherichia coli (range, 4.6 6.2 X 10(8) colony-forming units/ml). They then randomized into groups receiving either gentamicin (n = 10); pretreatment with muramyl dipeptide 11); or hemoperfusion through sham column 8), PMX-F-packed 8); 8). Thirty-three control received no treatment. Sham (13%) (21%) had lowest survival rate, although increased improvement was noted treated (30%) PMX-F filtration (50%). The most significant improvements occurred pretreated (53%) gentamicin, (88%). These data show nonspecific additive antibiotic therapy useful adjunctive measures multimodal treatment experimental gram-negative bacterial infection.