Flow Cytometric Sorting of Mammalian Sperm for Predetermination of Sex

摘要: The predetermination of sex is an appealing means controlling the ratio in a selected population production animals or wildlife. sexing mammalian sperm by flow cytometry for its use artificial insemination (AI) vitro fertilization (IVF) has led to birth hundreds thousands offspring numerous species, and remains only scientifically proven method selecting at time conception. Sperm made possible because difference total DNA content between carrying larger X chromosome, those smaller Y chromosome. To accomplish sorting, are stained with dye that allows cytometer detect approximate 4% sperm, separate them accordingly into two distinct populations purity 90%. Sex sorted bull routinely cryopreserved used AI, fertility retained approximately 70–80% achieved nonsorted sperm. A recent advance allowed sorting previously equal blastocyst formation bovine IVF even after recryopreservation. With particular reference dairy cattle, we discuss advantages determination, which sexed, effects process on viability fertility. various avenues sexed may be disseminated discussed, as current status sex-sorting from semen.