Characteristics of calves produced with sperm sexed by flow cytometry/cell sorting.

摘要: The objectives of this study were to determine whether calves produced by sexed sperm differed from controls and what extent the sex ratio was altered sexing procedure. Data collected 1,169 flow cytometry/cell sorting after staining with Hoechst 33342, 793 control during breeding trials between 1997 2001. Least squares ANOVA completed using factors treatment (sexed vs. sperm), 19 management groups 13 field trials, calf sex. Responses analyzed include gestation length, birth weight, calving ease, vigor, weaning abortion rate, death rates (neonatal through weaning). No significant difference observed for any response due or interactions (P > 0.10). Therefore, grew developed normally both pre- postnatally. A neurological disorder in four one farm. gross anatomical abnormalities reported study. Differences all responses among < 0.03 abortions P 0.01 other responses). Heifer bull 0.001) length (278.4 279.6 d), weight (32.8 35.2 kg), ease (1.15 1.30), (233 247 kg). Gestation did not affect characteristics calves. at unsexed 49.2% male. Sexing accuracy X-sorted 87.8% female calves, Y-sorted 92.1% male Flow can be used preselect safely approximately 90% accuracy.