The role of the gut microbiota in inflammatory diseases of childhood

摘要: The bacteria located within the human gastrointestinal tract (the gut microbiota) perform numerous protective, immunological and metabolic functions for host. They are increasingly implicated in pathogenesis of acquired inflammatory diseases childhood, namely: necrotising enterocolitis (NEC) bowel disease (Crohn’s (CD) ulcerative colitis (UC)). Study role that microbiota may play development such lead to new therapies modulate or even cure them. However many current techniques depend on ability study outwith their natural ecosystem. Although molecular can identify species independent standard cultures they enlighten little activity identified bacterial species, which be important propagation responses. Little is known about potential novel therapeutic strategies, as probiotics, NEC. In addition inadequate scientific rigour has been applied science probiotics. aims described this dissertation were test following hypotheses. Hypotheses: 1. Probiotics prevent NEC at risk infants very low birth weight (VLBW). 2. The labelled by stable isotope probing (SIP) measure activity. 3. Quantitative measurement unculturable a useful way studying changes microbiota, compared with measures diversity, our understanding bacterially mediated stimuli childhood.