Molecular cloning and characterization of avian sarcoma virus UR2 and comparison of its transforming sequence with those of other avian sarcoma viruses.

摘要: Avian sarcoma virus UR2 and its associated helper virus, UR2AV , were molecularly cloned into lambda gtWES X B by using unintegrated viral DNAs. One several clones obtained. The DNA was subsequently pBR322. Both DNAs tested for their biological activity transfection onto chicken embryo fibroblasts. When cotransfected with DNA, able to induce transformation of fibroblasts a morphology similar that parental . -specific protein kinase RNA detected in the transfected cells. Transforming ( ), produced from doubly Five six also shown be biologically active. insert clone is 3.4 kilobases length contains two copies long terminal repeat. Detailed restriction mapping showed shared 0.8 59 sequence, including portion gag, 1.4 39 env. transforming ros, ca. 1.2 kilobases. No significant homology found between v-ros conserved regions v-src, v-yes, or v- abl By contrast, v-fps. v-fps-related sequence mapped within 300-base-pair middle ros. Images