Cultivation of microvascular endothelial cells from human preputial skin.

摘要: A procedure is described for the isolation and cultivation of microvascular endothelium from human skin. Neonatal foreskins are pooled, washed, minced, dissociated by a mixture collagenase dispase. Microvascular endothelium, liberated in form intact capillary fragments, incompletely separated fibroblasts epidermal cells sieving through nylon mesh, followed velocity sedimentation on 5% bovine serum albumin. The endothelium-enriched fraction has been maintained primary culture up to 3 weeks. resulting epithelioid colonies have characterized morphologically both light transmission electron microscopy manifest all structural features that distinguish other, large-vessel endothelia culture. In addition, immunohistochemical studies using an indirect fluorescent antibody technique demonstrate these contain endothelium-specific product, Factor VIII antigen.