Hypo-osmolar stimulation of transepithelial Cl- secretion in cultured human T84 intestinal epithelial layers.
作者： G.T.A. McEwan , C.D.A. Brown , B.H. Hirst , N.L. Simmons
DOI: 10.1016/0167-4889(92)90135-X
关键词:
摘要: Abstract Intact epithelial monolayers of T 84 human colonic adenocarcinoma cells were exposed from the basolateral surfaces to hypo-osmotic media; in responsive tissues this resulted a transient stimulation inward short-circuit current (SCC) peak 12.9 ± 1.5 (S.E., n = 10) μA/cm 2 which declined prestimulation values SCC (2.1 ) within 5 min. Exposure media results an increase cytosolic [Ca 2+ ] i , dependent on extracellular Ca influx. The cell-swelling activated is abolished upon medium Cl − replacement and by 100 μM bumetanide applied basal-surfaces, consistent with resulting transepithelial secretion. DIDS (4,4′-diisothiocyanantostilbene-2,2′-disulphonic acid) also SCC; without effect VIP-stimulated SCC, suggesting distinct channels are involved two responses.
elsevier.com
sci-hub.se 参考文章(15)
N.L. Simmons, Tissue culture of established renal cell lines. Methods in Enzymology. ,vol. 191, pp. 426- 436 ,(1990) , 10.1016/0076-6879(90)91027-4
J.H. Widdicombe, J.J. Wine, The basic defect in cystic fibrosis Trends in Biochemical Sciences. ,vol. 16, pp. 474- 477 ,(1991) , 10.1016/0968-0004(91)90183-V
J.A. Tabcharani, W. Low, D. Elie, J.W. Hanrahan, Low-conductance chloride channel activated by cAMP in the epithelial cell line T84 FEBS Letters. ,vol. 270, pp. 157- 164 ,(1990) , 10.1016/0014-5793(90)81257-O
F. Giraldez, M.A. Valverde, F.V. Sepúlveda, Hypotonicity increases apical membrane Cl− conductance in Necturus enterocytes Biochimica et Biophysica Acta. ,vol. 942, pp. 353- 356 ,(1988) , 10.1016/0005-2736(88)90037-5
A Hazama, Y Okada, Ca2+ sensitivity of volume-regulatory K+ and Cl- channels in cultured human epithelial cells. The Journal of Physiology. ,vol. 402, pp. 687- 702 ,(1988) , 10.1113/JPHYSIOL.1988.SP017229
J Hunter, BH Hirst, NL Simmons, Epithelial secretion of vinblastine by human intestinal adenocarcinoma cell (HCT-8 and T84) layers expressing P-glycoprotein. British Journal of Cancer. ,vol. 64, pp. 437- 444 ,(1991) , 10.1038/BJC.1991.328
D.A Williams, F.S Fay, Intracellular calibration of the fluorescent calcium indicator Fura-2 Cell Calcium. ,vol. 11, pp. 75- 83 ,(1990) , 10.1016/0143-4160(90)90061-X
A M Hurst, M Hunter, Stretch‐activated channels in single early distal tubule cells of the frog. The Journal of Physiology. ,vol. 430, pp. 13- 24 ,(1990) , 10.1113/JPHYSIOL.1990.SP018278
J D McCann, M Li, M J Welsh, Identification and regulation of whole-cell chloride currents in airway epithelium. The Journal of General Physiology. ,vol. 94, pp. 1015- 1036 ,(1989) , 10.1085/JGP.94.6.1015
K Dharmsathaphorn, K G Mandel, H Masui, J A McRoberts, Vasoactive intestinal polypeptide-induced chloride secretion by a colonic epithelial cell line. Direct participation of a basolaterally localized Na+,K+,Cl- cotransport system. Journal of Clinical Investigation. ,vol. 75, pp. 462- 471 ,(1985) , 10.1172/JCI111721