Overexpression of diacylglycerol acyltransferase in Yarrowia lipolytica affects lipid body size, number and distribution.

摘要: In the oleaginous yeast Yarrowia lipolytica, diacylglycerol acyltransferases (DGATs) are major factors for triacylglycerol (TAG) synthesis. The Q4 strain, in which four have been deleted, is unable to accumulate lipids and form lipid bodies (LBs). However, expression of a single acyltransferase this strain restores TAG accumulation LB formation. Using system, it becomes possible characterize activity specificity an individual DGAT. Here, we examined effects DGAT overexpression on formation Y. lipolytica Specifically, evaluated consequences introducing one or two copies genes YlDGA1 YlDGA2 Overall, multi-copy increased content cells. size distribution LBs depended specific overexpressed. caused large LBs, while generated smaller but more numerous LBs. This phenotype was accentuated through addition second copy overexpressed gene might be linked distinct subcellular localization each DGAT, i.e. YlDga1 being localized YlDga2 structure strongly resembling endoplasmic reticulum.