Sensitive Detection and Conjoint Analysis of Promoter Methylation by Conjugated Polymers for Differential Diagnosis and Prognosis of Glioma.

摘要: Glioma is the most common primary tumor in central nervous system (CNS) with worst prognosis. Accurate pathological diagnosis has always been a challenge for optimal management of glioma. Promoter methylation an important mechanism epigenetic silencing tumor-suppressor genes and potential biomarker differential Herein, using cationic conjugated polymer (CCP)-based fluorescence resonance energy transfer (FRET) technique, we realized highly sensitive detection promoter clinical samples minimal degree (1.25%) trace DNA quantity (10 ng/μL). Results three glioma-related (MGMT, CDKN2A, TERT) were combined diagnostic classifier to analyze glioma-CpG island methylator phenotype (G-CIMP), which achieved sensitivity 80% at maximum specificity 100% glioma diagnosis. Kaplan-Meier survival curves Pearson correlation analysis revealed that prognosis patients high G-CIMP scores (>5) was significantly better than those low scores, especially diffuse midline astrocytoma. This CCP-based FRET technique determining status could provide rapid reasonably accurate glioma, as well valuable prognostic prediction can guide individual treatment.