Role of diffusion in nonaqueous enzymology. 1. Theory.

摘要: Using a set of standard equations, we have calculated the role internal and external mass transfer in limiting rate enzyme-catalysed reactions anhydrous organic solvents supercritical fluids. We shown that enzyme particles suspended will be subject to increasing diffusional limitation as activity particle size increase. dimensions, measured by scanning electron microscopy, prepared series graphs enable investigators determine whether their combination result or limitations. fluids can expected enhance enzymes nonaqueous environments high diffusivity bulk solvent. The plots also clearly indicate require nearly two orders magnitude less agitation than those conventional order overcome any