Metabolism of n-Butyl Benzyl Phthalate in the Female Wistar Rat. Identification of New Metabolites

摘要: n-Butyl benzyl phthalate (BBP), a plasticizer used in polyvinylchloride (PVC) and other polymers, has been orally administered to female Wistar rats with four doses (150, 475, 780 1500 mg/kg body weight/day) for 3 consecutive days. Metabolites recovered urines were analysed by gas chromatography-mass spectrometry (GC-MS) after 24, 48 72 hours. Six metabolites identified. Mono-n-butyl (MBuP) mono-n-benzyl (MBeP) represented respectively 29-34% 7-12% of the total metabolites. Hippuric acid, main metabolite benzoic second major (51-56%). Phthalic acid an omega-oxidized MBuP also urine but small quantities. BBP was never identified urines. Total urinary recovery 56% dose first 24 However, decreased when increases (43% at weight/day, only 30% weight/day). Whatever time was, all present same proportions two lowest doses. Discrepancy quantities expressed as percentages observed rat treated highest disappeared MBuP, MBeP hippuric significantly increased day 3. Metabolic profile established. The aim study is identify further active(s) agent(s) involved malformations teratogenic effects.