Comparative microarray analysis of microRNA expression profiles in primary cutaneous malignant melanoma, cutaneous malignant melanoma metastases, and benign melanocytic nevi

摘要: Perturbations in microRNA (miRNA) expression profiles have been reported for cutaneous malignant melanoma (CMM) predominantly when examined cell lines. Despite the rapidly growing number of newly discovered human miRNA sequences, availability up-to-date clinical samples primary (PCMM), metastases (CMMM), and benign melanocytic nevi (BMN) is limited. Specimens excised from center tumors (lesional) patients with PCMM (n=9), CMMM (n=4), or BMN (n=8) were obtained during surgery. An exploratory microarray analysis was performed by profiling based on Agilent platform screening 1205 miRNAs. The results validated TaqMan quantitative real-time polymerase chain reaction. In addition to several miRNAs previously known be associated CMM, 19 unidentified candidates found dysregulated CMM patient samples. Among novel candidates, genes hsa-miR-22, hsa-miR-130b, hsa-miR-146b-5p, hsa-miR-223, hsa-miR-301a, hsa-miR-484, hsa-miR-663, hsa-miR-720, hsa-miR-1260, hsa-miR-1274a, hsa-miR-1274b, hsa-miR-3663-3p, hsa-miR-4281, hsa-miR-4286 upregulated, hsa-miR-24-1*, hsa-miR-26a, hsa-miR-4291, hsa-miR-4317, hsa-miR-4324 downregulated. this study partially confirm previous studies identifying that are CMM. However, we report tumors; these sequences require further validation functional evaluate whether they play a role pathogenesis