Physical state of cytochrome oxidase: Relationship between membrane formation and ionic strength

作者: F.F. Sun , K.S. Prezbindowski , F.L. Crane , E.E. Jacobs

DOI: 10.1016/0005-2728(68)90008-X

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摘要: Abstract Preparations of lipid-containing and lipid-depleted cytochrome oxidase from beef heart mitochondria through use Triton non-ionic detergents is described. The shown to form either a membranous or dispersed state. state requires the presence salt whereas condition obtained by reducing ionic strength. shows low activity in comparison form. Typical unit membrane structure can be sections reaggregated even though lipid content as eight percent. Both show 50-A globules negative staining lipid-free strings 20 A diameter.

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