作者: Ciro César Rossi , Ingrid da Silva Dias , Igor Mansur Muniz , Walter Lilenbaum , Marcia Giambiagi-deMarval
DOI: 10.1016/J.VETMIC.2017.01.029
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摘要: This study aimed to characterize the species, antimicrobial resistance and dispersion of CRISPR systems in staphylococci isolated from oropharynx domestic cats Brazil. Staphylococcus strains (n = 75) were identified by MALDI-TOF sequencing rpoB tuf genes. Antimicrobial susceptibility was assessed disk diffusion method PCR investigate presence antimicrobial-resistance genes usually present mobile genetic elements (plasmids), addition plasmid extraction. − arrangements that give bacteria ability resist entry exogenous DNA investigated essential protein Cas1 gene. A great diversity species (n = 13) identified. The understudied like S. nepalensis pettenkoferi reveals more than one identification may be necessary achieve conclusive results. At least 56% contain plamids, being 99% resistant at eight tested antimicrobials 12% multidrug resistant. rare among studied strains, consistent with their putative role as gene reservoirs. Moreover, herein we describe for first time existence lentus, which system must confer additional adaptive advantage. Prevalence against used veterinary human clinical practice zoonotic risk highlight need better management practices, transfer themselves, including virulent aureus.