First assessment of classical swine fever marker vaccine candidate CP7_E2alf for oral immunization of wild boar under field conditions

摘要: Oral vaccination against classical swine fever (CSF) is a potent tool to control disease outbreaks in wild boar. So far, campaigns have been carried out using live attenuated vaccines that do not allow serological differentiation of infected from vaccinated animals (DIVA). Although this drawback acceptable for boar, the use marker would facilitate studies on and dynamics. Recently, CSF vaccine candidate CP7_E2alf was assessed oral immunization under laboratory conditions. Promising results prompted efforts study field conditions bait formulation. In context, two were with areas called ‘faunistic-hunting farms’ region Umbria, Italy. One campaign conducted single vaccination, second routinely employed double strategy. Both before concerted hunting actions performed. Bait uptake, virus detection antibody responses along inspections upon gutting. As comparator, seven boar hand-fed baits field, uptake ranged 63.7% 98.7%, whereas prevalence reached only 33.3–35.1%. The serology showed strong influence sample quality test outcome total 85% samples being classified correctly. Vaccine detectable. Under hand feeding conditions, six took up at least one bait, five them detectable levels weeks after vaccination. These supplemented by stability tests. Appropriate shown both total, most line our expectations. However, optimization DIVA assay has be attempted future.