CP7_E2alf: a safe and efficient marker vaccine strain for oral immunisation of wild boar against Classical swine fever virus (CSFV).

摘要: Wild boar are an important reservoir of Classical swine fever virus (CSFV) in several European countries, where most the primary outbreaks domestic pigs directly related to endemic disease situation wild population. Oral immunisation has been introduced as additional control measure accelerate CSF eradication Germany since 1993. Immunisation with oral bait vaccine based on conventionally attenuated live strain "C" proved be safe and effective, but does not allow differentiation between infected vaccinated animals. Therefore, we examined efficacy recently constructed chimeric pestivirus CP7_E2alf, whose coding sequences for major envelope protein E2 BVDV CP7 replaced by CSFV Alfort187 [Reimann I, Depner K, Trapp S, Beer M. An avirulent altered cell tropism protects against lethal infection classical virus. Virology 2004;322(1):143-57]. Following boar, CP7_E2alf completely avirulent. Furthermore, all vaccinees were fully protected from clinical after a highly virulent challenge infection. The immunised animals seroconverted within 3 weeks vaccination E2-specific neutralising antibodies, whereas prior no antibodies E(rns) detected appropriate CSFV-specific marker ELISA test. Thus, backbone enables serological genetic type In conclusion, represents first efficient candidate CSFV.