Comparison of proteoglycans extracted from high and low weight-bearing human articular cartilage, with particular reference to sialic acid content.

摘要: Proteoglycan subunits extracted in the presence of proteinase inhibitors from autologous knee and shoulder cartilage were examined different donors various ages, order to determine effect weight bearing on age-related changes proteoglycan structure. At each age, high density proteoglycans two joints same individual virtually identical with respect chemical composition, hydrodynamic size, ability aggregate hyaluronic acid, chondroitin sulfate chain length. The content decreased as did size subunits. In contrast, proportions keratan sulfate, protein glycosaminoglycan, 6 4-sulfate all increased age. These occurred extent both cartilage. There was no change acid. sialic acid profile Bio-Gel P-10 chromatography, following alkaline borohydride degradation, showed oligosaccharide chains. core appeared decrease during aging process, but it could not be ascertained whether this related abundance sulfate.