The use of caesium sulphate density gradient centrifugatin to analyse proteoglycans from human articular cartilages of different ages

摘要: Abstract Proteoglycan subunits from human articular cartilage were fractionated by caesium sulphate density gradient centrifugation. A single heterogeneous population of molecules was produced whose average decreased with increasing age the individual which they obtained. At no did carbohydrate composition any adult fraction resemble that newborn fraction, although there considerable overlap in density. However, a similarity amino acid between most dense proteoglycans and those least newborn. The content 2-year-old proteoglycan intermediate composition, high fractions resembling low adult. In addition, lowest both two year preparations showed additional bands agarose/polyacrylamide gel electrophoresis material. These results indicate while core protein may occur juvenile it need to necessarily be glycosylated an manner, suggesting glycosylation is some extent independent origin heterogeneity.