[120] Sedimentation equilibrium in a buoyant density gradient

摘要: Publisher Summary This chapter describes the sedimentation equilibrium in a buoyant density gradient. Equilibrium ultracentrifugation gradient is method for study of macromolecules, viruses, and particulate materials. A homogeneous mixture concentrated binary solvent dilute macrospecies are brought to sedimentation-diffusion ultracentrifuge. The new distribution presents stable At same time concentrates form band redistributed solvent. Density experiments performed either preparative or analytical ultracentrifuges. concentration distributions at independent shapes containers. It found that when accurate interest, approximately optimum quantities must be introduced so as obtain with accuracy. has also been observed pressure dependence position results from difference compressibility apparent macrospecies, variation solvation salt concentration.