Monoclonal antibody analysis of Listeria monocytogenes-induced cytotoxic lymphocytes.

摘要: An immunizing infection with Listeria monocytogenes provides a potent stimulus for the formation of prekiller lymphocytes. Their cytolytic potential is revealed when cells are restimulated in vitro by antigens. monocytogenes-induced cytotoxic lymphocytes and from which they derived were characterized respect to their surface antigenic markers. Using monoclonal antibodies, B-cell depleted thoracic duct immune rats fractionated into subsets combination panning sorting techniques. cell precursors demonstrated have phenotype W3/25-, OX8+, OX4+, W3/13+ (high density), OX19+ (low RT6.1-. The RT6.1- subset, contained cells, constituted approximately 6% lymph-borne T cells. data indicate that these microbial antigen-induced belong minor subset peripheral whose properties distinguish them natural killer