Microsomal DPNH cytochrome c reductase.
作者： Nathar Penn , Bruce Mackler
DOI: 10.1016/0006-3002(58)90383-4
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摘要: Publisher Summary This chapter discusses the microsomal DPNH-cytochrome c reductase. Enzymatic activity at 38° was measured spectrophotometrically by following rate of reduction cytochrome 550 mμ. Protein concentration is determined biuret method, with deoxycholate added to a final 1%. All procedures are carried out 0-5°. Beef liver obtained fresh from slaughterhouse diced and ground in an electric meat grinder. The stages purification procedure described. preparations slowly lose when stored 0° or frozen at –20°, approximately 50% being lost over period 1-2 weeks. Stage II particle suspensions did not show TPNH-cytochrome e reductase, aldehyde oxidase, xanthine succinic dehydrogenase activity. enzyme actively reduces ferricyanide DPNH as substrate. Preparations highest purity contain one mole flavin, 2 moles b 5 atom nonheme iron per enzyme. has approximate molecular weight 1.5×10 6 including lipid, which constitutes 70% dry
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