Regulation of System A amino-acid transport activity by phospholipase C and cAMP-inducing agents in skeletal muscle: Modulation of insulin action

摘要: The present study was designed to investigate the effect of phospholipase C and compounds known promote synthesis cAMP on System A transport activity under basal insulin-stimulated conditions in incubated muscle. In parallel, we also examined these agents muscle glucose activity. Phospholipase caused marked stimulation alpha-(methyl)-aminoisobutyric acid (MeAIB--a System-A-specific analogue) uptake that 3-O-methylglucose by contrast, activatory insulin largely inhibited C. effects processes differed from provoked phorbol esters (TPA), indicating they are not just a consequence TPA-sensitive protein kinase activation. Agents such as isoproterenol, cholera toxin or forskolin, inducers, glycogen depletion lactate production However, did alter MeAIB uptake. Isoproterenol affect maximal insulin. Our data indicate is activated skeletal muscle, this due simply activation isoforms reduced either TPA, which suggests mediation On basis lack cAMP-inducing activities, conclude cAMP-dependent does cause any generalized blockade action contrast what has been reported other cell types.