Determination of oocyte membrane permeability coefficients and their application to cryopreservation in a rabbit model.

摘要: Abstract Having an effective means to cryopreserve human oocytes would offer more flexibility in healthcare services for infertility patients, and obviate cryopreservation of preimplantation embryos. It is essential establish good animal models oocyte the rabbit a candidate. Attempts improve are often empirical, with results being irreproducible. Cryopreservation protocols may be optimized by modeling changes volume associated damages incurred during addition dilution cryoprotective agents (CPA). The objectives current study were determine cryobiological properties oocytes, including isotonic volume, osmotically inactive cell fraction ( V b ), hydraulic conductivity L p permeability P s ) dimethylsulfoxide (Me 2 SO), ethylene glycol (EG), glycerol (GLY) examine correlation between excursions viability. This has led development accumulative osmotic damage (AOD) model processes CPA addition/dilution. Mature perfused 15% (V/V) medium (dissolved 1× PBS). responses videotaped. A two-parameter was fit experimental data values . Oocyte volumes reached upon equilibration 285, 600, 900, 1200 mOsm (milliosmolal) solutions non-permeating compounds plotted Boyle van’t Hoff plot. average radius solution determined 55.7 ± 1.2 μm n  = 16). exhibited “ideal” response range from iso-osmolity 1200 mOsm. 20% value r  = 0.97. 0.79 ± 0.26, 0.82 ± 0.22, 0.64 ± 0.16 μm min −1  atm 2.9 ± 1.3, 2.7 ± 1.3, 0.27 ± 0.18 × 10 −3  cm min Me SO, EG GLY, respectively. There no significant differences  > 0.05) S presence SO EG. However, these significantly different GLY. We calculated AOD those that experienced process additions/dilutions found highly correlated rates after parthenogenetic activation  = −0.98).