Screening of Insect Cell Lines for the Production of Recombinant Proteins and Infectious Virus in the Baculovirus Expression System

摘要: Eight cell lines derived from the insects Spodoptera frugiperda, Trichoplusia ni, Mamestra brassicae, and Estigmene acrea were evaluated for recombinant β-galac-tosidase infectious virus production following infection with baculovirus Autographa californica multiple nuclear polyhedrosis (AcMNPV). Production was assessed on a specific (per per microgram of uninfected cellular protein) volumetric milliliter) basis. Cell density found to be an important factor in comparing due density-dependent inhibition protein that appeared result cell-cell contact. After cells at low-density β-galactosidase would drop between β- 6-fold five eight when plated tissue culture plates near-confluent confluent densities. The Sf 21 9 least sensitive density. accounting effects differences size, two lines, BTI Tn 5B1-4 TnM, identified superior other including 9, production. Optimal TnM 2-fold 5-fold higher, respectively, both than optimal or cells. line also had highest viability all 3 days postinfection could adapted serum-free media. Specific varied by less after size taken into account. No correlation NOV although decrease similarly increasing