Characterization of growth and recombinant protein production of the insect cell line BTI-Tn-5Bl-4 (High Five™) in suspension culture
作者： Ernesto Chico , Anja Kobold , Bernd Haase , Volker Jäger
DOI: 10.1007/978-94-011-5404-8_120
关键词: Cell culture 、 Suspension culture 、 Laboratory flask 、 Glutamine 、 Line (text file) 、 Recombinant protein production 、 Cell biology 、 Insect cell 、 Chemistry 、 Asparagine
摘要: In this work, the insect cell line BTI-Tn-5Bl-4 (High Five™), normally anchorage dependent, was adapted to grow in suspension by a subculturing procedure without supplementation of heparin. Comparative testing different serum-free media were carried out batch culture using spinner flasks. Oxygen, asparagine and glutamine identified as limiting nutrients growth ExCell 401 medium. A recombinant baculovirus, encoding for human immunoglobulin G, used infection High Five™ cells during study production capability.
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