Strategies to Increase the Efficiency of Membrane Aerated and Perfused Animal Cell Bioreactors by an Improved Medium Perfusion
作者： H. D. Blasey , V. Jäger
DOI: 10.1007/978-94-011-3550-4_8
关键词:
摘要: A bioreactor system based on internal membrane aeration and perfusion was used to cultivate a variety of mammalian insect cell lines for the production recombinant proteins, monoclonal antibodies or biomass. The efficiency this reactor is evaluated with respect maximum concentration, productivity lifetime in order give some fundamental data comparison other stirred tank bioreactors. Due capacities perfusion, eight thirty fold increases concentration compared batch cultivation culture flasks could be obtained routinely unchanged viabilities.
springer.com
sci-hub.st 参考文章(17)
Mona Lucki-Lange, Roland Wagner, CONDITIONS FOR THE PRODUCTION OF RECOMBINANT IL-2 IN STIRRED SUSPENSION CULTURE USING A PROTEIN FREE MEDIUM Production of Biologicals from Animal Cells in Culture. pp. 180- 186 ,(1991) , 10.1016/B978-0-7506-1103-9.50044-5
E. Rebsamen, W. Goldinger, W. Scheirer, O.-W. Merton, G.E. Pàlfi, Use of a dynamic filtration method for separation of animal cells. Developments in biological standardization. ,vol. 66, pp. 273- 277 ,(1987) , 10.1016/B978-0-408-02732-8.50046-8
R. E. Spier, J. B. Griffiths, Animal Cell Biotechnology ,(1988)
A Mizrahi, Pluronic polyols in human lymphocyte cell line cultures. Journal of Clinical Microbiology. ,vol. 2, pp. 11- 13 ,(1975) , 10.1128/JCM.2.1.11-13.1975
Horst D. Blasey, Ursula Winzer, Low protein serum-free medium for antibody-production in stirred bioreactors Biotechnology Letters. ,vol. 11, pp. 455- 460 ,(1989) , 10.1007/BF01026641
A.G. Fane, C.J.D. Fell, K.J. Kim, The effect of surfactant pretreatment on the ultrafiltration of proteins Desalination. ,vol. 53, pp. 37- 55 ,(1985) , 10.1016/0011-9164(85)85051-7
Hansjörg Eibl, William E.M. Lands, A new, sensitive determination of phosphate Analytical Biochemistry. ,vol. 30, pp. 51- 57 ,(1969) , 10.1016/0003-2697(69)90372-8
Bridget T. Hill, S. Whatley, A simple, rapid microassay for DNA FEBS Letters. ,vol. 56, pp. 20- 23 ,(1975) , 10.1016/0014-5793(75)80102-5
P. Himmelfarb, P. S. Thayer, H. E. Martin, Spin Filter Culture: The Propagation of Mammalian Cells in Suspension Science. ,vol. 164, pp. 555- 557 ,(1969) , 10.1126/SCIENCE.164.3879.555
William R. Tolbert, Joseph Peder, Richard C. Kimes, Large-scale rotating filter perfusion system for high-density growth of mammalian suspension cultures. In Vitro Cellular & Developmental Biology – Plant. ,vol. 17, pp. 885- 890 ,(1981) , 10.1007/BF02618284