Multi-Host Expression System for Recombinant Production of Challenging Proteins

摘要: Recombinant production of complex eukaryotic proteins for structural analyses typically requires a profound screening process to identify suitable constructs the expression ample amounts properly folded protein. Furthermore, evaluation an optimal host has major impact on protein yield and quality as well actual cost process. Here we present novel fast system multiple hosts based single donor vector termed pFlp-Bac-to-Mam. The range applications pFlp-Bac-to-Mam comprises highly efficient transient transfection HEK293-6E in serum-free suspension culture subsequent large-scale challenging expressing mg per Liter level using either baculoviral or stable CHO cell lines generated by Flp-mediated cassette exchange. success multi-host strategy high is demonstrated comparative study three model representing different classes: intracellular fluorescent protein, secretion single-chain-Fv-hIgG1Fc fusion construct large amount homogeneous sample extracellular domain Toll-like receptor. efficiency shows that allows individual optimization each class.