HIGH DENSITY INSECT CELL CULTURE FOR THE PRODUCTION OF RECOMBINANT PROTEINS WITH THE BACULOVIRUS EXPRESSION SYSTEM

摘要: Abstract IPLB-Sf21-AE cells (Spodoptera frugiperda) were propagated in stirred tank perfusion bioreactors. A maximum cell density of 5.5×107 viable ml−1 was achieved during continuous cultivation the mode with medium exchange rates up to 4 reactor volumes per day. Usually, infected after reaching a concentration 1 3×107 ml−1. The secreted product harvested continuously, together spent free and debris. In addition, more than 99.9 % baculovirus retained within bioreactor could be used for subsequent infection cells. Therefore, can an extremely low multiplicity (MOI) which usually kept at 0.02. Maximum volumetric production recombinant proteins (e.g. human IL-2 glycoforms) 5 6 days post infection, one two before viability dropped drastically because virus induced lysis. Compared batch cultures there only slight increase productivity. However, rapidly removed from bioreactor, thus reducing degradations by enzymes lysed it has been shown that not uniform phase. contrast cultures, several harvests containing different microheterogeneous forms protein separated.