Comparison of impedimetric detection of DNA hybridization on chemically and electrochemically functionalized multi-wall carbon nanotubes modified electrode
作者： Ali Benvidi , Nooshin Rajabzadeh , Mohammad Mazloum-Ardakani , Mohammad M. Heidari
DOI: 10.1016/J.SNB.2014.10.043
关键词:
摘要: Abstract A label-free DNA biosensor was constructed on the base of multi-walled carbon nanotube (MWCNTs) modified electrode. This paper compares detection Amylogenin using a glassy electrode (GCE) with chemically and electrochemically functionalized MWCNTs. Functionalized MWCNTs were used to improve sensitivity detection. The structure characterized by UV–Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR) scanning electron microscopy (SEM). optimum conditions found for immobilization probe onto surface its hybridization target in both methods. cyclic voltammetry (CV) electrochemical impedance (EIS). Hybridization corresponding complementary ssDNA is monitored changes charge transfer resistance ( R ct ). also shows good linear relationship between logarithm concentration ranging from 1.0 × 10 −17 M −12 correlation coefficient 0.9961 −18 −13 0.9994 results show that method better functionalization than chemical method. In addition two methods satisfactorily applied sequence real sample DNA.
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