Electrochemically reduced graphene oxide supported poly(indole-5-carboxylic acid) nanocomposite for genosensing application
作者： Wei Zhang
DOI: 10.1039/C5RA21071B
关键词: Dielectric spectroscopy 、 Organic chemistry 、 Conjugated system 、 Chemistry 、 Carboxylic acid 、 Graphene 、 Substrate (chemistry) 、 Combinatorial chemistry 、 Nanocomposite 、 Biosensor 、 Nucleobase
摘要: In this paper, the poly(indole-5-carboxylic acid) (PICA) was electropolymerized on a pre-obtained electrochemically reduced graphene oxide (ERGNO) membrane to develop novel direct DNA electrochemical biosensor. Due unique properties of graphene, conjugated indole-5-carboxylic acid (ICA) monomers tended be adsorbed plane by π–π* stacking and electropolymerization efficiency greatly improved, resulting in an enhanced response PICA. The obtained PICA/ERGNO nanocomposite not only served as substrate for immobilization but also reflected transduction originating from hybridization without any complicated labeling or outer indicators. probe ssDNA successfully conducted via noncovalent assembly ascribed interaction between bases. After with target DNA, formation double-helix structure induced resulted dsDNA released surface nanocomposite, accompanied self-signal regeneration (“signal-on”). dynamic range biosensor detecting complementary BCR/ABL fusion gene chronic myelogenous leukemia 1.0 × 10−16 10−9 mol L−1 using impedance spectroscopy, detection limit 2.3 10−17 L−1.
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