Routine phenotyping of native and desialyzed α2HS-glycoprotein from blood stains

摘要: The application of a polyacrylamide gel isoelectric focusing (PAGIEF) and immunoblotting procedure for the identification native alpha 2HS-glycoprotein (AHSG) in routine casework blood stains has produced reportable results on 57.2% samples. This reporting rate is lower than that group specific component (GC) (83.8%) phosphoglucomutase (PGM 1) (72.8%) phenotyping same Blood stain samples were desialyzed with 1 U/ml neuraminidase, overnight at room temperature prior to PAGIEF gels containing pharmalyte pH 5-6 2.5 M urea. Simple AHSG patterns developed by immunoblotting. was five times as sensitive method desialyzation reproducible over range incubation neuraminidase concentrations. analysis resulted significant increase number (762 reported out 1027 samples). (74.2%) compares favourably GC (79.1%) PGH (69.6%) three alleles (AHSG*1, 2 3) are clearly resolved after sample separation sensitivity approaches this technique worthy inclusion screening protocols forensic laboratories regions where population limited rare alleles.