Nuclear‐Magnetic‐Resonance‐Spectroscopic Studies of the Amino Groups of Insulin
作者： J. Howard BRADBURY , Larry R. BROWN
DOI: 10.1111/J.1432-1033.1977.TB11627.X
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摘要: The amino groups of insulin have been studied by 1H and 13C nuclear magnetic resonance spectroscopy in insulin, zinc-free methylated insulin. By difference it is possible to follow the shift with pH epsilon-CH2 delta-CH2 proton resonances lysine-B29 In dimethyl glycine-A1, phenylalanine-B1 can be followed as a function pH. native pKapp values 6.7 8.0 are obtained for glycine-A1 (the assignment tentative) 11.2 lysine-B29. Separate observed from Nepsilon-(CH3)2 group monomeric dimeric forms which indicates small change environment on dimerisation. spectral characteristics these are, general, consistent overall structure crystal form 2-zinc hexamer.
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