Immunolocalization of choline acetyltransferase of common type in the central brain mass of Octopus vulgaris.

摘要: Acetylcholine, the first neurotransmitter to be identified in vertebrate frog, is widely distributed among animal kingdom. The presence of a large amount acetylcholine nervous system cephalopods well known from several biochemical and physiological studies. However, little about precise distribution cholinergic structures due lack suitable histochemical technique for detecting acetylcholine. most reliable method visualize neurons immunohistochemical localization enzyme choline acetyltransferase, synthetic Following our previous study on patterns Octopus vulgaris visual system, using novel antibody that recognizes acetyltransferase common type (cChAT), now we extend investigation octopus central brain mass. When applied sections ganglia, immunoreactivity cChAT was detected cell bodies all mass lobes with notable exception subfrontal subvertical lobes. Positive varicosed nerves fibers where observed neuropil