Structure–function studies of the functional and binding epitope of the human 37 kDa laminin receptor precursor protein

摘要: Expression of the 37 kDa laminin receptor precursor protein (37LRP) correlates directly with increased invasiveness and metastatic potential tumors. The 37LRP matures to a 67 which facilitates binding cancer cells basement membranes. palindrome peptide sequence LMWWML, corresponding 173-178-residue stretch human sequence, has been identified as laminin-1-binding site. Peptides from species that contain this palindrome-bind laminin-1 high affinity. Nuclear magnetic resonance (NMR) conformational studies have undertaken on synthetic 15-residue (KGAHSVGLMWWMLAR) containing establish structural basis activity. To further correlate data function, analogous were conducted for similar (RGKHSIGLIWYLLAR) lacking palindrome, originating Saccharomyces cerevisiae exhibiting low Finally, in vitro cell invasion assays performed investigate possibility affinity peptides influences their inhibitory