Binding of shrimp cellular proteins to Taura syndrome viral capsid proteins VP1, VP2 and VP3.

作者: Saengchan Senapin , Amornrat Phongdara

DOI: 10.1016/J.VIRUSRES.2006.06.013

关键词:

摘要: Viruses are a major cause of production losses in the world shrimp-farming industry. Despite this, little is known about viral-host interactions shrimp due part to lack continuous cell lines. Here, yeast two-hybrid assay system was employed study between three Taura syndrome viral capsid proteins (VP1-VP3) and from cDNA library black tiger Penaeus monodon. VP1 interacted with beta-actin, elongation factor 1alpha (EF1alpha), lysozyme (Lys) laminin receptor/ribosomal protein p40 (Lamr/p40) containing putative palindromic binding region LMWWML. VP2 beta-actin EF1alpha, while VP3 bound same as except for Lamr/p40. In vitro pull-down assays confirmed these interactions. The most interesting interaction specific Lamr/p40 since has been identified mammalian receptor several arthropod-borne viruses (arboviruses). A search mosquito vector Drosophila sequences at available databases revealed presence high homology shrimp.

参考文章(57)
Herve Lesot, Uwe Kühl, Klaus von der Mark, Isolation of a laminin-binding protein from muscle cell membranes The EMBO Journal. ,vol. 2, pp. 861- 865 ,(1983) , 10.1002/J.1460-2075.1983.TB01514.X
Chun-I Yu, Yen-Ling Song, Outbreaks of Taura Syndrome in Pacific White Shrimp Penaeus vannamei Cultured in Taiwan. Fish Pathology. ,vol. 35, pp. 21- 24 ,(2000) , 10.3147/JSFP.35.21
S N Konavalova, E V Protopopova, V B Loktev, [Isolation of a cellular receptor for tick-borne encephalitis virus using anti-idiotypic antibodies]. Voprosy virusologii. ,vol. 42, pp. 264- 268 ,(1997)
V. Castronovo, G. Taraboletti, M.E. Sobel, Functional domains of the 67-kDa laminin receptor precursor. Journal of Biological Chemistry. ,vol. 266, pp. 20440- 20446 ,(1991) , 10.1016/S0021-9258(18)54943-7
Erguang Li, Dwayne Stupack, Gary M. Bokoch, Glen R. Nemerow, Adenovirus Endocytosis Requires Actin Cytoskeleton Reorganization Mediated by Rho Family GTPases Journal of Virology. ,vol. 72, pp. 8806- 8812 ,(1998) , 10.1128/JVI.72.11.8806-8812.1998
David C. Amberg, Daniel Burke, Jeffrey N. Strathern, Methods in yeast genetics : a Cold Spring Harbor Laboratory course manual Cold Spring Harbor Laboratory Press. ,(2005)
M. Jaseja, L. Mergen, K. Gillette, K. Forbes, I. Sehgal, V. Copié, Structure–function studies of the functional and binding epitope of the human 37 kDa laminin receptor precursor protein Journal of Peptide Research. ,vol. 66, pp. 9- 18 ,(2008) , 10.1111/J.1399-3011.2005.00267.X
S Ou, J H Strauss, K S Wang, R J Kuhn, E G Strauss, High-affinity laminin receptor is a receptor for Sindbis virus in mammalian cells. Journal of Virology. ,vol. 66, pp. 4992- 5001 ,(1992) , 10.1128/JVI.66.8.4992-5001.1992