Constitutive and induced expression of DC-SIGN on dendritic cell and macrophage subpopulations in situ and in vitro.

摘要: DC-SIGN is a C-type lectin, highly expressed on the surface of immature dendritic cells (DCs), that mediates efficient infection T in trans by its ability to bind HIV-1, HIV-2, and SIV. In addition, adhesion molecules surfaces naive endothelium also suggests involvement T-cell activation DC trafficking. To gain further insights into range expression potential functions DC-SIGN, we performed detailed analysis adult fetal tissues analyzed regulated cultured DCs macrophages. First, show restricted subsets specialized macrophages placenta lung. There were no overt differences between except alveolar was only present after birth. Similarly, tissues, observed primarily (CD83-negative) DCs. Secondly, peripheral blood, found small subset BDCA-2+ plasmacytoid precursors (pDC2), concordant with our finding large numbers DC-SIGN-positive allergic nasal polyps (previously shown be infiltrated DC2). Triple-label confocal microscopy indicated colocalized BDCA-2 CD123 polyp tissue. Consistent this observation can up-regulated monocyte-derived upon exposure Th2 cytokine, IL-13. summary, data demonstrate relevant populations express vivo where it may impact efficiency virus indicate involved axis immunity.