Equilibrium saturation chromatographic method for studying the binding of ligands to human serum albumin by high-performance liquid chromatography. Influence of fatty acids and sodium dodecyl sulphate on warfarin-human serum albumin binding.
作者： Bernard Sebille , Nicole Thuaud , Jean Paul Tillement
DOI: 10.1016/S0021-9673(00)80178-8
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摘要: Abstract A size exclusion chromatographic method for studying ligand-macromolecule binding parameters is described. This equilibrium saturation allows the determination of concentrations constituents in and specially useful characterizing ligand-protein under conditions that can be compared with physiological conditions. The has been used measuring warfarin-human serum albumin (HSA) influence free fatty acids (FFA) sodium dodecyl sulphate on warfarin-HSA binding. Some comparisons Hummel Dreyer are given. FFA strongly dependent their chain length, an inversion effect a 10-carbon chain.
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