DNA Analysis Confirms Macroptilium lathyroides as Alternative Host of Bean golden yellow mosaic virus
作者： V. Bracero , L. I. Rivera , J. S. Beaver
DOI: 10.1094/PDIS.2003.87.9.1022
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摘要: ABSTRACT The leguminous weed Macroptilium lathyroides is considered a potential host of the Bean golden yellow mosaic virus (BGYMV; BGMV = Mesoamerican isolates). To determine if M. could be for BGYMV, an infectivity cycle was established between this and Phaseolus vulgaris. Virus transmission carried out using whitefly, Bemisia argentifolli, as vector. Inoculated plants both species were examined symptoms such mosaic, stunting, leaf distortion. P. vulgaris showed during all stages. Symptomatic tested BGYMV polymerase chain reaction (PCR) nucleotide sequence analysis. Two degenerate primers sets used PCR to detect viral DNA: PAL1v1978/PAR1c715 PCRc2/PBL12039. analysis PCRc2/PBL12039 amplified DNA component B from plant species. Nucleotide revealed 93% identity isolated...
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