Anti-smooth muscle antibodies (ASMAs) and anti-cytoskeleton antibodies (ACTAs) in liver diseases: a comparison of classical indirect immunofluorescence with ELISA

摘要: In the diagnosis of autoimmune hepatitis type I (AIH-I), routine assay indirect immunofluorescence (IFL), used for detection anti-smooth muscle antibodies (ASMAs), has a low predictive value. On other hand, enzyme-linked immunosorbent (ELISA), which detects anti-cytoskeleton (ACTAs), presents contradictory results concerning their specific antigenic target. this study, we first looked immunological properties (isotypes and targets) autoantibodies in AIH-I two control liver diseases: primary biliary cirrhosis (PBC) viral (VH), using ELISA based on cytoskeleton proteins: F-actin, G-actin, myosin, tropomyosin, troponin, desmin, vimentin, keratin, an extract HEp-2 carcinoma cells. We also compared diagnostic value IFL ELISA. contrast to previous studies, found that actin was not AIH-I. No autoantigen no antibody class or subclass discriminated from diseases. is more suitable diagnosis, as 97% sera but only 22% PBC were ASMA-positive. Additionally, 96% ASMA-positive, all ASMA-negative three diseases ACTA-positive. ASMA mainly IgG, while >50% ACTA contained IgA IgM. These data suggest ACTAs recognize additional epitopes ASMAs, they frequently occur