Fine specificity and subclasses of IgG anti-actin autoantibodies differ in health and disease

摘要: Current opinions suggest that autoantibodies occurring in autoimmune diseases are generated by B-cells which primarily produce polyspecific natural autoantibodies, through either polyclonal activation or specific antigen selection of these B-cells. In this study, we compared the immunological properties (polyspecificity, fine specificity and IgG subclasses) between anti-actin antibodies (N-AAA) disease-associated AAA (D-AAA). from sera healthy donors, patients with hepatitis type 1 (AIH-1) primary biliary cirrhosis (PBC) were affinity-purified on actin immunoadsorbent tested initially for polyspecificity against various cytoskeleton proteins enzyme-linked immunosorbent assay (ELISA). Fine was studied Western blotting using proteolytic peptides ELISA synthetic 12 mer peptides, spanning 221-377 aa sequence actin. Results showed both N-AAA D-AAA polyspecific. Nevertheless, a reactivity pattern as to N-AAA, 16 kDa C-terminal (229-377 aa) peptide more specifically P36 (351-362 aa). Quantitation subclasses revealed IgG1 IgG3 increased AIH-1 PBC, respectively, N-AAA. We conclude differentiated terms subclasses, probably producing