Phytoestrogen interaction with estrogen receptors in human breast cancer cells.

摘要: The interactions of phytoestrogens with estrogen receptors were studied in the human breast cancer cell line, MCF-7. compounds tested coumestrol, genistein, and formononetin mycotoxins, zearalenone its reduced derivative, zearalenol. All but compete for binding [3H]-estradiol to unfilled cytoplasmic receptor or nuclear sites. Relative affinities are zearalenol HMP (high melting point isomer) greater than LMP (low = coumestrol genistein formononetin. Dissociation constants estimated from competition curves show that high. In contrast estradiol, bind only weakly sex steroid-binding globulin; they also do not corticosteroid-binding globulin. These translocate whole cells. Bound then processed a manner similar estradiol step which rapidly decreases total cellular receptors. biologically active; can markedly enhance tumor proliferation. sum, interact cells culture and, therefore, may affect estrogen-mediated events these