Molecular epidemiology of malaria in Cameroon. XXVII. Clinical and parasitological response to sulfadoxine-pyrimethamine treatment and Plasmodium falciparum dihydrofolate reductase and dihydropteroate synthase alleles in Cameroonian children.

摘要: The rapidly changing epidemiology of antifolate-resistant Plasmodium falciparum in Africa requires monitoring. present study was designed to assess the degree association between clinical and parasitological response sulfadoxine-pyrimethamine allelic combinations dihydrofolate reductase (dhfr) dihydropteroate synthase (dhps) genes. Of 357 children who completed 14-day follow-up, an adequate observed 316 patients (88.5%) early late failures occurred 18 (5%) 23 (6.4%, mostly due recrudescence) patients, respectively. majority isolates were characterized as "quadruple" (n=196, 55.2%; N51I-C59R-S108N DHFR A437G DHPS) or "triple" mutants (n=97, 27.3%; wild-type DHPS; S108N+N51I C59R DHPS). Wild-type, single mutation, double mutation 29, 20, 13 parasites, comparison different sets mutations did not reveal any molecular marker associated with treatment outcome when follow-up period limited 14 days (P>0.05). In this study, determination dhfr-dhps genotypes value predict individual few haplotypes. Further monitoring will be required define relationship SP therapy parasite our epidemiological setting.