Kenyan Plasmodium falciparum Field Isolates: Correlation between Pyrimethamine and Chlorcycloguanil Activity In Vitro and Point Mutations in the Dihydrofolate Reductase Domain
作者： A. Nzila-Mounda , E. K. Mberu , C. H. Sibley , C. V. Plowe , P. A. Winstanley
DOI: 10.1128/AAC.42.1.164
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摘要: Sixty-nine Kenyan Plasmodium falciparum field isolates were tested in vitro against pyrimethamine (PM), chlorcycloguanil (CCG), sulfadoxine (SD), and dapsone (DDS), their dihydrofolate reductase (DHFR) genotypes determined. The data show that CCG is more potent than PM DDS SD. DHFR genotype correlated with drug response. Isolates can be classified into three distinct groups based on 50% inhibitory concentrations (IC 50 s) for ( P s of 3.71 ± 6.94 0.24 0.21 nM, respectively. second group includes parasites which all have mutations at codon 108 alone or also codons 51 59 represents one homogeneous 25- 6-fold increases IC s, respectively, are observed. Parasites 108, 51, (triple mutants) form a third nine- eightfold compared to the Surprisingly, there significant decrease
sci-hub.se 参考文章(54)
C. D. K. Bottema, S. S. Sommer, A. R. Groszbach, PCR amplification of specific alleles (PASA) is a general method for rapidly detecting known single-base changes. BioTechniques. ,vol. 12, pp. 82- 87 ,(1992)
H. C. Spencer, W. M. Watkins, J. D. Chulay, D. G. Sixsmith, D. K. Koech, A new in vitro test for pyrimethamine/sulfadoxine susceptibility of Plasmodium falciparum and its correlation with in vivo resistance in Kenya. Bulletin of The World Health Organization. ,vol. 62, pp. 615- 621 ,(1984)
Sherwan Zindrou, Gote Swedberg, Pham Thi Xuyen, Nguyen Duy Sy, Le Duc Dao, Nguyen Phuong Dung, Ola Skold, Rapid detection of pyrimethamine susceptibility of Plasmodium falciparum by restriction endonuclease digestion of the dihydrofolate reductase gene. American Journal of Tropical Medicine and Hygiene. ,vol. 54, pp. 185- 188 ,(1996) , 10.4269/AJTMH.1996.54.185
Christopher V. Plowe, Abdoulaye Djimde, Madama Bouare, Ogobara Doumbo, Thomas E. Wellems, Pyrimethamine and Proguanil Resistance-Conferring Mutations in Plasmodium falciparum Dihydrofolate Reductase: Polymerase Chain Reaction Methods for Surveillance in Africa American Journal of Tropical Medicine and Hygiene. ,vol. 52, pp. 565- 568 ,(1995) , 10.4269/AJTMH.1995.52.565
Geoffrey Pasvol, Charles R. J. C. Newton, Kevin Marsh, David A. Warrell, Joab B. O. Were, Norbert M. Peshu, William M. Watkins, Peter A. Winstanley, Quinine treatment of severe falciparum malaria in African children: a randomized comparison of three regimens. American Journal of Tropical Medicine and Hygiene. ,vol. 45, pp. 702- 713 ,(1991) , 10.4269/AJTMH.1991.45.702
Walter H. Wernsdorfer, David Payne, The dynamics of drug resistance in Plasmodium falciparum Pharmacology & Therapeutics. ,vol. 50, pp. 95- 121 ,(1991) , 10.1016/0163-7258(91)90074-V
W Trager, J. Jensen, Human malaria parasites in continuous culture Science. ,vol. 193, pp. 673- 675 ,(1976) , 10.1126/SCIENCE.781840
P. B. Bloland, E. M. Lackritz, P. N. Kazembe, J. B. O. Were, R. Steketee, C. C. Campbell, Beyond Chloroquine: Implications of Drug Resistance for Evaluating Malaria Therapy Efficacy and Treatment Policy in Africa The Journal of Infectious Diseases. ,vol. 167, pp. 932- 937 ,(1993) , 10.1093/INFDIS/167.4.932
Jason M. Wooden, Leland H. Hartwell, Brandi Vasquez, Carol Hopkins Sibley, ANALYSIS IN YEAST OF ANTIMALARIA DRUGS THAT TARGET THE DIHYDROFOLATE REDUCTASE OF PLASMODIUM FALCIPARUM Molecular and Biochemical Parasitology. ,vol. 85, pp. 25- 40 ,(1997) , 10.1016/S0166-6851(96)02808-3
D. S. Peterson, W. K. Milhous, T. E. Wellems, Molecular basis of differential resistance to cycloguanil and pyrimethamine in Plasmodium falciparum malaria. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 87, pp. 3018- 3022 ,(1990) , 10.1073/PNAS.87.8.3018