A Wnt-BMP4 Signaling Axis Induces MSX and NOTCH Proteins and Promotes Growth Suppression and Differentiation in Neuroblastoma.
作者： Marianna Szemes , Zsombor Melegh , Jacob Bellamy , Alexander Greenhough , Madhu Kollareddy
DOI: 10.3390/CELLS9030783
关键词:
摘要: The Wnt and bone morphogenetic protein (BMP) signaling pathways are known to be crucial in the development of neural crest lineages, including sympathetic nervous system. Surprisingly, their role paediatric neuroblastoma, prototypic tumor arising from this lineage, remains relatively uncharacterised. We previously demonstrated that Wnt/β-catenin can have cell-type-specific effects on neuroblastoma phenotypes, growth inhibition differentiation, BMP4 mRNA were induced by Wnt3a/Rspo2. In study, we characterised phenotypic cells, demonstrating convergent induction MSX homeobox transcription factors BMP4-induced suppression differentiation. An immunohistochemical analysis expression primary neuroblastomas confirms a striking absence poorly differentiated tumors, contrast high ganglion cells. These results consistent with suppressive for neuroblastoma. RNA sequencing following treatment revealed Notch signaling, verified increases Notch3 Hes1 proteins. Together, our data demonstrate, first time, Wnt-BMP-Notch crosstalk associated
mdpi.com
scilit.net参考文章(46)
Roel Nusse, Hans Clevers, Wnt/β-Catenin Signaling, Disease, and Emerging Therapeutic Modalities. Cell. ,vol. 169, pp. 985- 999 ,(2017) , 10.1016/J.CELL.2017.05.016
Tim van Groningen, Jan Koster, Linda J Valentijn, Danny A Zwijnenburg, Nurdan Akogul, Nancy E Hasselt, Marloes Broekmans, Franciska Haneveld, Natalia E Nowakowska, Johannes Bras, Carel J M van Noesel, Aldo Jongejan, Antoine H van Kampen, Linda Koster, Frank Baas, Lianne van Dijk-Kerkhoven, Margriet Huizer-Smit, Maria C Lecca, Alvin Chan, Arjan Lakeman, Piet Molenaar, Richard Volckmann, Ellen M Westerhout, Mohamed Hamdi, Peter G van Sluis, Marli E Ebus, Jan J Molenaar, Godelieve A Tytgat, Bart A Westerman, Johan van Nes, Rogier Versteeg, Neuroblastoma is composed of two super-enhancer-associated differentiation states Nature Genetics. ,vol. 49, pp. 1261- 1266 ,(2017) , 10.1038/NG.3899
Isabelle Westerlund, Yao Shi, Konstantinos Toskas, Stuart M. Fell, Shuijie Li, Olga Surova, Erik Södersten, Per Kogner, Ulrika Nyman, Susanne Schlisio, Johan Holmberg, Combined epigenetic and differentiation-based treatment inhibits neuroblastoma tumor growth and links HIF2α to tumor suppression Proceedings of the National Academy of Sciences of the United States of America. ,vol. 114, pp. 201700655- ,(2017) , 10.1073/PNAS.1700655114
Valentina Boeva, Caroline Louis-Brennetot, Agathe Peltier, Simon Durand, Cécile Pierre-Eugène, Virginie Raynal, Heather C Etchevers, Sophie Thomas, Alban Lermine, Estelle Daudigeos-Dubus, Birgit Geoerger, Martin F Orth, Thomas GP Grünewald, Elise Diaz, Bertrand Ducos, Didier Surdez, Angel M Carcaboso, Irina Medvedeva, Thomas Deller, Valerie Combaret, Eve Lapouble, Gaelle Pierron, Sandrine Grossetete-Lalami, Sylvain Baulande, Gudrun Schleiermacher, Emmanuel Barillot, Hermann Rohrer, Olivier Delattre, Isabelle Janoueix-Lerosey, None, Heterogeneity of neuroblastoma cell identity defined by transcriptional circuitries Nature Genetics. ,vol. 49, pp. 1408- 1413 ,(2017) , 10.1038/NG.3921
Megan L. Martik, Marianne E. Bronner, Regulatory Logic Underlying Diversification of the Neural Crest Trends in Genetics. ,vol. 33, pp. 715- 727 ,(2017) , 10.1016/J.TIG.2017.07.015
Fraua Christina Ferlemann, Vishal Menon, Alexandra Larisa Condurat, Jochen Rößler, Jan Pruszak, Surface marker profiling of SH-SY5Y cells enables small molecule screens identifying BMP4 as a modulator of neuroblastoma differentiation. Scientific Reports. ,vol. 7, pp. 13612- 13612 ,(2017) , 10.1038/S41598-017-13497-8
Duc-Hiep Bach, Hyen Joo Park, Sang Kook Lee, The Dual Role of Bone Morphogenetic Proteins in Cancer Molecular Therapy - Oncolytics. ,vol. 8, pp. 1- 13 ,(2018) , 10.1016/J.OMTO.2017.10.002
Marc-Antoine Lauzon, Nathalie Faucheux, A small peptide derived from BMP-9 can increase the effect of bFGF and NGF on SH-SY5Y cells differentiation Molecular and Cellular Neuroscience. ,vol. 88, pp. 83- 92 ,(2018) , 10.1016/J.MCN.2018.01.003
Marianna Szemes, Alexander Greenhough, Zsombor Melegh, Sally Malik, Aysen Yuksel, Daniel Catchpoole, Kelli Gallacher, Madhu Kollareddy, Ji Hyun Park, Karim Malik, Wnt Signalling Drives Context-Dependent Differentiation or Proliferation in Neuroblastoma. Neoplasia. ,vol. 20, pp. 335- 350 ,(2018) , 10.1016/J.NEO.2018.01.009
Marianna Szemes, Alexander Greenhough, Karim Malik, Wnt Signaling Is a Major Determinant of Neuroblastoma Cell Lineages Frontiers in Molecular Neuroscience. ,vol. 12, pp. 90- ,(2019) , 10.3389/FNMOL.2019.00090